Glutathione Reductase Activity

Glutathione Reductase Activity

Product name: Glutathione Reductase (GR) Assay Kit

Detection method: Colorimetric

Example type: Urine, serum, plasma, other biological fluids, tissue extracts, cell lysate

Test type: Enzymatic activity

Sensitivity: > 0.1 mU / ml

Distance: 0.1 mU / ml – 40 mU / ml

Test time: 0h 40m

Product description

The Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct, and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5′-dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml of GR in various samples.

Notes

Glutathione reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH / GSSG ratio is essential for protection against oxidative stress.

Relevance

Glutathione reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH / GSSG ratio is essential for protection against oxidative stress.

Cell location: Cytoplasmic and mitochondrial

Alternative names

  • EC 1.8.1.7
  • Mitochondrial glutathione reductase
  • GRAM

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